Fig. 6

ICOSaa partially reverses mechanical allodynia in female SNI mice. A Schematic representation of groups subjected to SNI on day one followed by intrathecal injection of ICOSaa on days 14–17. Arrows represent days with von Frey testing. B Mechanical hypersensitivity was partially reversed only on days 17 and 19 in the group administered with ICOSaa group (two-way ANOVA, F = 3.473, p-value < 0.0001, post hoc Bonferroni, ***SNI vs. SNI + ICOSaa at day 17, p-value = 0.0006, ***SNI vs. SNI + ICOSaa at day 19, p-value = 0.0003), N = 7/group. C Effect size was determined by calculating the cumulative difference between the value for each time point and the baseline value. The effect size was significant between SNI and SNI + ICOSaa-treated mice (one-way ANOVA, F = 18.0, p-value < 0.0001, post hoc Tukey, Sham vs. SNI + ICOSaa, p-value = 0.0265, Sham vs. SNI, p = value < 0.0001, SNI vs. SNI + ICOS, p-value = 0.0151). ***p < 0.001