Fig. 1

The dynamic alterations of Tregs after LPC injection and depletion of Tregs aggravates lymphocytes infiltration as well as microgliosis. A Representative images of infiltration of CD45⁺ leukocytes and CD3⁺Foxp3⁺ Tregs in CC at 10 dpi of sham and LPC group. CC in sham group and lesions in LPC group were indicated by dashed line. Enlarged images of Tregs infiltration were shown in right panel, and Tregs were indicated by white arrows. Scale bar = 50 μm. B–D Quantification of CD45⁺ leukocytes (B), CD3⁺ T lymphocytes (C) and Tregs (D) in CC at 10 dpi of sham and LPC group. E Representative immunofluorescent images of CD45⁺ leukocytes, CD3⁺ T lymphocytes infiltration and staining of Iba1 and CD68 in CC at 10 dpi in PBS-treated and DT-treated LPC group were shown, demyelinated lesions were indicated by dashed line, scale bar = 50 μm. F, G Statistical analysis of CD45⁺ leukocytes (F) and CD3⁺ T lymphocytes (G) in lesions of LPC group treated by PBS and DT. H, I Quantification of Iba1⁺ area (H) and CD68⁺ area (I) in lesions of LPC group treated by PBS and DT. Two-tailed Student’s t-test was used, n = 5/group. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001