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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-κB pathway in LPC-induced demyelination

Fig. 2

Depletion of Tregs exacerbates myelin injury in LPC-induced demyelination. A Representative confocal images of NG2+ OPCs and GST-Pi+ mature oligodendrocytes in sham group and LPC-demyelination group treated with PBS and DT, respectively. CC in sham group and lesions in LPC group were indicated by dashed line. Enlarged views of square area were shown. Scale bar = 50 μm. B Quantification of NG2 positive area in lesions of sham and LPC group treated with PBS and DT. C Statistical analysis of GST-Pi+ mature oligodendrocytes in lesions in PBS and DT-treated sham or LPC-induced demyelinated mice. D, E Representative LFB staining of CC in different groups (D) and quantification of demyelinated areas (E). Demyelinated areas were indicated by dashed black lines. Scale bar = 200 μm. F Representative images of western blot of MBP, NF-H, NF-M, NF-L and β-actin among groups. G-J Quantification of protein expressions of MBP (G), NF-H (H), NF-M (I) and NF-L (J) in sham and LPC-demyelination group treated by PBS and DT. N = 5/group. Two-way ANOVA with Tukey’s post hoc test was used. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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