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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-κB pathway in LPC-induced demyelination

Fig. 4

Caspase-1 inhibitor VX765 significantly decreases pyroptosis of microglia which is enhanced by Tregs ablation. A Confocal images of Iba1 co-staining with caspase-1, GSDMD and IL-1β in sham and LPC group treated by PBS, DT plus vehicle and DT plus VX765 were shown, CC in sham group and lesions in LPC group were indicated by dashed line, magnified views were displayed. Scale bar = 50 μm. BD Caspase-1-positive microglia per mm2 (B), GSDMD-positive microglia per mm2 (C) and IL-1β-positive microglia per high-power field (HPF) (D) in lesions were quantified. N = 5 for each group. EI Representative images for western blot of pyroptosis-related proteins NLRP3, GSDMD, cleaved GSDMD and cleaved caspase-1 in sham and LPC-demyelination group treated by PBS, DT with vehicle and DT with VX765 (E), statistical analyses of corresponding protein expressions were shown in F-I, respectively. N = 6/group. One-way ANOVA followed by Tukey’s post hoc test was used. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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