Fig. 5
Caspase-1 inhibitor VX765 partly reverses myelin injury aggravated by Tregs depletion. A Representative confocal images of GST-Pi+ mature oligodendrocytes in lesions in sham and LPC group with different treatments. CC in sham group and lesions in LPC group were outlined by dashed white lines, and enlarged images were displayed in the down panel. Scale bar = 50 μm. B LFB staining of CC was shown among groups. Demyelinated areas were indicated by dashed black lines. Scale bar = 200 μm. C Quantification of GST-Pi+ mature oligodendrocytes per mm2 within lesions in LPC-demyelinated mice treated by PBS, DT + vehicle and DT + VX765. N = 5/group. D Quantification of demyelinated areas of LFB staining. N = 5/group. E-I Western blot of NF-H, NF-M, NF-L and MBP in sham and LPC group treated by PBS, DT plus vehicle and DT plus VX765 (E), corresponding statistical analysis for protein expressions of NF-H (F), NF-M (G), NF-L (H) and MBP (I) were shown. N = 6 for each group. One-way ANOVA with Tukey’s test was applied. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001