Fig. 7

RNA-sequencing analysis reveals that TLR4/MyD88 play central roles in pyroptosis exacerbated by Tregs depletion in LPC-induced demyelination. A Venn diagram of DEGs between Sham VS PBS-LPC and PBS-LPC VS DT-LPC. KEGG pathway enrichment analysis of common DEGs was shown in the right panel. B Heatmap of hierarchical clustering analysis of DEGs involved in Nod-like receptor signaling pathway. DEGs appeared in D and E were marked in red. C GSEA analysis of Nod-like receptor signaling pathway. D, E PPI (D) and KDA (E) analysis of Nod-like receptor signaling pathway were shown, with the top ten DEGs indicated. For RNA-sequencing analysis, n = 5 replicates per group. F–J Representative images of western blot of NLRP3, p-NF-κB, NF-κB, MyD88 and cleaved caspase-1 (F), corresponding statistical analysis for protein expressions of NLRP3 (G), p-NF-κB/NF-κB (H), MyD88 (I) and cleaved caspase-1 (J) were shown. N = 5–6/group, one-way ANOVA with Bonferroni’s test was applied. *P < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001