Fig. 2

Mitochondrial networks are severely affected by Aβ accumulation. The cells were labeled with CellLight Mitochondria-GFP followed by ICC in order to visualize the mitochondrial networks (green), Aβ (red) and the cell nuclei (blue) (A). MiNa ImageJ macro was used to analyze the networks and the images were skeletonized for optimal analysis (B). The quantifications showed that there was a decrease in the number of mitochondria in Aβ-exposed astrocytes at day 7d + 6d, but an increase at 7d + 12d (C). Motility analysis, using Mitometer, revealed that mitochondria displacement (D) and distance (E) were increased in Aβ-exposed astrocytes. Scale bar: A = 20 µm