Fig. 3

Aβ accumulation causes mitochondrial fission and fusion dysfunction. TEM analysis demonstrated that while control astrocytes had healthy mitochondria profiles at 7d + 6d (A), astrocytes exposed to Aβ displayed mitochondria profiles that were extremely long (B) and abnormally branched (B’). At 7d + 12, the mitochondria profiles in the control cultures still showed a healthy morphology (C), while in the Aβ-exposed astrocytes had swollen (D) and very short (E) mitochondria profiles. Quantifications confirmed that mitochondria profiles in Aβ-exposed astrocytes were significantly longer than in control astrocytes at 7d + 6d, but significantly shorter at 7d + 12d (F). The aspect ratio between mitochondria length and width showed the same pattern (G)