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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Amyloid-β accumulation in human astrocytes induces mitochondrial disruption and changed energy metabolism

Fig. 5

Astrocytes transfer pDRP-1 through EVs and TNT. TEM showed EVs in close proximity to the astrocytes in controls (A) and Aβ-exposed cultures (B). EVs were visualized with TEM after isolation through ultracentrifugation (C). WB analysis of the isolated EVs demonstrated that pDRP-1 (S616) (D) and pDRP-1 (S637) (E) are secreted from astrocytes, also showing a significant increase of pDRP-1 (S637) after Aβ exposure. However, the levels of the mitochondrial marker VDAC-1 were stable (F). ICC stainings of Aβ-exposed astrocytes from 7d showed the presence of pDRP-1 (S616) in both thicker protrusions (white arrowhead) (G) and in thin TNTs (G’ and white arrowheads in G”) connecting the cells. Scale bar: 20 µm

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