Table 2 Overview of primary antibodies
Antibody | Host | Technique | Target | Company | Cat # |
|---|---|---|---|---|---|
Vimentin | Chicken | Immunocytochemistry (1:500) | Astrocytes | Sigma Aldrich | AB5733 |
GLAST-1 | Rabbit | Immunocytochemistry (1:400) | Astrocytes | Novus Biologicals | NB100-1869 |
AQP4 | Rabbit | Immunocytochemistry (1:200) | Astrocytes | Novus Biologicals | NBP1-87679 |
GFAP | Chicken | Immunocytochemistry (1:400) | Astrocytes | Abcam | ab4674 |
Nestin | Rabbit | Immunocytochemistry (1:200) | Astrocytes | Millipore | ABD69 |
COX IV | Rabbit | Immunocytochemistry (1:200), WB (1:500) | Mitochondria | Abcam | ab14744 |
VDAC-1 | Mouse | Immunocytochemistry (1:200), WB (1:500) | Mitochondria | Abcam | ab14734 |
pDRP-1 (S616) | Rabbit | Immunocytochemistry (1:200), WB (1:500) | Mitochondrial dynamic | Cell signaling | 4494S |
pDPR-1 (S637) | Rabbit | Immunocytochemistry (1:200), WB (1:500) | Mitochondrial dynamic | Abcam | ab193216 |
DRP-1 | Mouse | Immunocytochemistry (1:200), WB (1:500) | Mitochondrial dynamic | Abcam | ab56788 |
Catalase | Rabbit | Immunocytochemistry (1:200), WB (1:500) | Peroxisomes | Abcam | ab16731 |
PMP70 | Mouse | Immunocytochemistry (1:200), WB (1:500) | Peroxisomes | Abcam | ab211533 |
Flotillin1 | Rabbit | Immunocytochemistry (1:500), WB (1:500) | Extracellular vesicles | Abcam | ab41927 |
S100β | Mouse | Immunocytochemistry (1:1000) | Astrocytes | Sigma Aldrich | S2532 |
OPA1 | Mouse | WB (1:200) | Mitochondrial dynamic | Beckman Dickinson | 612606 |
BAX | Rabbit | WB (1:200) | Apoptosis | Abcam | ab199677 |
LDH | Rabbit | Immunocytochemistry (1:200), WB (1:500) | Glycolysis | Abcam | ab75167 |
Caspase-3 | Rabbit | WB (1:500) | Apoptosis | Abcam | ab4051 |
GAPDH | Mouse | WB (1:5000) | Loading control | Abcam | ab8245 |