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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Activation of endogenous retrovirus triggers microglial immuno-inflammation and contributes to negative emotional behaviors in mice with chronic stress

Fig. 2

Antiretroviral therapy Entecavir reversed chronic stress-induced microglial morphological activation, ERVs transcription, intrinsic nucleic acids sensing response, and inflammation in BLA. A Representative immunofluorescent images showing Iba1+CD68+ microglia in BLA, as well as statistical analysis of the number of Iba1+ microglia and Iba1+CD68+ microglia in BLA. One-way ANOVA with Tukey's post hoc test, ***P < 0.01, compared with CONT; ###P < 0.001, compared with SUS-PBS; scale bar = 40 × ; a total of 21 replicates from n = 3. B Representative immunofluorescent and their 3D reconstructive Sholl images showing Iba1+ microglia in BLA, statistical analysis of microglial area and cell body area of Iba1+ microglia in BLA, as well as Sholl analysis of Iba1+ microglia in BLA. One-way ANOVA for areas analysis and repeated measure ANOVA for Sholl analysis with Tukey's post hoc test, ***P < 0.01, compared with CONT; ##P < 0.01, ###P < 0.001, compared with SUS-PBS; scale bar = 60 × ; a total of 90 replicates from n = 3. C Representative flow cytometry strategy for gating CD11b+CD45Low microglia in BLA. D Representative flow cytometry images for showing CD11b+CD45LowMHC-II+ microglia in BLA, as well as statistical analysis of percentage of CD11b+CD45LowMHC-II+ microglia in BLA. One-way ANOVA with Tukey's post hoc test, ***P < 0.001, compared with CONT; ###P < 0.001, compared with SUS-PBS; n = 6. E Statistical analysis of murine orthologous microglial ERVs genes expression in BLA. One-way ANOVA with Tukey's post hoc test, $$$P < 0.01, compared with ET; #P < 0.05, ##P < 0.01, ###P < 0.001, compared with SUS-PBS; n = 3. F Representative western blot images for showing microglial p-p53, p53, IFI16, STING, p-p65, p65, NLRP3, caspase-1, IL-1β, and β-actin in BLA and cortex, as well as their statistical analysis of relative proteins expression. One-way ANOVA with Tukey's post hoc test, *P < 0.05, ***P < 0.001, compared with CONT; #P < 0.05, ##P < 0.01, ###P < 0.001, compared with SUS-PBS; n = 3

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