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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Cannabinoids modulate the microbiota–gut–brain axis in HIV/SIV infection by reducing neuroinflammation and dysbiosis while concurrently elevating endocannabinoid and indole-3-propionate levels

Fig. 2

Chronic THC administration increased WFS1 protein expression in the basal ganglia of chronically SIV-infected RMs. Basal ganglia tissues of uninfected control (A), VEH/SIV (B–D), and THC/SIV RMs (E–G) were immunostained for WFS1 (green), NeuN (red), and DAPI for nuclear staining (blue). Note the significantly decreased WFS1 (B–D) staining in the BG of VEH/SIV RMs. In contrast, WFS1 (E–G) staining is intense in NeuN+ neurons in the BG of THC/SIV RMs. Representative immunofluorescence images were captured using a Zeiss confocal microscope at 20X magnification. Yellow staining (A, E, F) indicates colocalization of WFS1 to NeuN+ neurons (white arrow). A few NeuN− cells expressing WFS1 protein were also detected (B, C, and F, white arrowhead). Quantitation of WFS1 (H) signal intensity was performed using Halo software. Differences in WFS1 signal intensity between groups were analyzed using unpaired “t” tests after confirming data assumptions (normal distribution) employing the Prism v9 software (GraphPad software). A p-value of < 0.05 was considered significant

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Journal of Neuroinflammation

ISSN: 1742-2094