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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Cannabinoids modulate the microbiota–gut–brain axis in HIV/SIV infection by reducing neuroinflammation and dysbiosis while concurrently elevating endocannabinoid and indole-3-propionate levels

Fig. 3

Chronic THC administration increased CRYM protein expression in the basal ganglia of chronically SIV-infected RMs. Basal ganglia tissues of uninfected control (A), VEH/SIV (B–D), and THC/SIV RMs (E–G) were immunostained for CRYM (green), NeuN (red), and DAPI for nuclear staining (blue). Note the significantly increased CRYM staining in NeuN+ neurons in the BG of THC/SIV (E–G) compared to VEH/SIV (B–D) and uninfected control RMs (A). A few NeuN− cells expressing CRYM protein were also detected (E and G, white arrowhead). Representative immunofluorescence images were captured using a Zeiss confocal microscope at 20X magnification. Yellow staining (A, E) indicates colocalization of CRYM to NeuN+ neurons (white arrow). Quantitation of CRYM (H) signal intensity was performed using Halo software. Differences in CRYM signal intensity between groups were analyzed using unpaired “t” tests after confirming data assumptions (normal distribution) employing the Prism v9 software (GraphPad software). A p-value of < 0.05 was considered significant

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Journal of Neuroinflammation

ISSN: 1742-2094