Fig. 1

Subretinal fibrosis in young and aged mice. A Schematic view of the study design. Mice received two laser burns 10 days apart, eyes were collected 30 days after the second laser for immunohistochemistry. B Representative confocal images of RPE/choroid flatmounts stained for collagen-1 from young (2.5-month) and aged (15–16-month) mice. C Quantitative analysis of collagen-1⁺ lesion area in young and aged group. D, E Representative confocal images of retinal cryosections from young (D) and aged mice (E) with subretinal fibrosis stained for CD31 (green) and collagen-III (red). Cell nuclei were stained with DAPI (blue). F Percentage of CD31⁺ blood vessel area in collagen-III⁺ fibrotic lesions of young and aged mice. Mean ± SD, n = 30–40 lesions per group from 8 to 10 eyes in C, n = 22 lesions per group from 5 to 6 eyes, ***p < 0.001, Student t test. Scale bar = 50 µm