Fig. 6

Macrophage to myofibroblast transition (MMT) in the subretinal fibrotic lesion in young and aged mice. A–D Representative confocal image of retinal cryosections from young (2.5-month, A, B) and aged (15–16-month, C, D) mice with subretinal fibrosis stained for F4/80 (green) and collagen-1 (red). Cell nuclei were stained with DAPI (blue). Images in B and D showing magnified area of boxes in A and C. Arrows indicating F4/80⁺collagen-1⁺ cells. Scale bar = 50 µm in (A and C); and 20 µm in B, D. E Number of F4/80⁺ cells in subretinal fibrotic lesion of young and aged mice. F Percentage of F4/80⁺collagen-1⁺ cells among all F4/80⁺ cells in subretinal fibrotic lesions of young and aged mice. Mean ± SD, n = 18–20 lesions per group from 5 to 6 eyes, **p < 0.01, Student t test