Fig. 1

ZIKV infection alters lipid metabolism in human neuroblastoma cells. A Viral replication was analyzed by PFU assay in SH-SY5Y cells after 24 h and 48 h of ZIKV infection at an MOI of 1. B Immunofluorescence analyses of SH-SY5Y cells after ZIKV infection at an MOI of 1 for 48 h. The double strain RNA was detected by indirect immunofluorescence with a J2 antibody (red), and the nuclei were stained with DAPI (blue). The scale bar represents 20 µm in range. C Images of SH-SY5Y cells infected with ZIKV at MOIs of 0.1, 1, and 5 after 48 h. The cells were stained with Oil Red O (Red) and DAPI (blue) for nuclei. The scale bar represents 10 µm in range. D Quantification of the fluorescence area per cell in SH-SY5Y cells. E Real-time PCR for proteins related to lipid metabolism after 24 h of ZIKV infection. F Representative western blot analysis and densitometry data set of PPAR-gamma (G) and the lipolytic enzymes ATGL and HSL in SH-SY5Y lysates 48 h post ZIKV infection. Data information: In A, D–F, data are presented as the means ± SEMs of three independent experiments. *P < 0.05 mock- versus ZIKV-infected cells