Fig. 2
From: Metabolic reprogramming and lipid droplets are involved in Zika virus replication in neural cells
ZIKV infection modulates lipid metabolism in human neural stem cells. A Viral replication was analyzed by PFU assay in NSCs after 24 h and 48 h of ZIKV infection at an MOI of 1. B Images of NSCs infected with ZIKV at MOIs of 0.1, 1, and 5 after 48 h. The cells were stained with Oil Red O (Red) and DAPI (blue) for nuclei. The scale bar represents 10 µm in range. C Quantification of the fluorescence area per cell in NSCs. D Representative western blot analysis of PPAR-gamma, (E) precursor and mature SREBP-1, (F) ATGL and HSL in NSC lysates 48 h post-ZIKV infection at an MOI of 1. Data information: In A, C-D, data are presented as the means ± SEMs of three independent experiments. *P < 0.05 mock- versus ZIKV-infected cells