Fig. 4
From: Metabolic reprogramming and lipid droplets are involved in Zika virus replication in neural cells
Upregulation of LD biogenesis increased ZIKV replication in SH-SY5Y cells. A Representative images of SH-SY5Y cells treated with 40 µM oleic acid and 50 µM DGAT-1 inhibitor (A922500) 48 h after ZIKV infection. The ds-RNA was stained with a J2 antibody (green), the lipid droplets were stained with LipidTox (red), and the nuclei were stained with DAPI (blue). The scale bar represents 10 µm in range. B Quantification of LD pixels per cell. C Quantification of ds-RNA pixels per cell. D Effect of the DGAT-1 inhibitor on ZIKV replication at 48 hpi. Data information: In A–C, data are presented as the means ± SEMs of three independent experiments, and in D, data are presented as the means ± SEMs of four independent experiments. *P < 0.05 Veh alone versus Veh with oleic acid-treated cells. #P < 0.05 A922500 alone versus A922500 with oleic acid-treated cells