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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Metabolic reprogramming and lipid droplets are involved in Zika virus replication in neural cells

Fig. 6

Treatment with DGAT-1 inhibitor (A922500, iDGAT) reduces viral loads in the brain during acute infection, increases survival, and inhibits weight loss in ZIKV-infected mice. A Three-day-old Swiss mice were infected with Brazilian ZIKV (2 × 104 PFU) and treated with iDGAT-1 one day before infection for 7 days. On the indicated days after infection, animals were euthanized. B ZIKV RNA levels were measured in the brain. C Weight variation (D) and survival were assessed during treatment. E Inflammatory mediators were measured in mouse brain extracts by ELISA: TNF, IL-1β and MCP-1. Data information: In B, the viral loads are displayed as the mean ± SEM of seven ZIKV-infected mice, and twelve ZIKV-infected mice per day were assayed. Student's t test was used to compare the viral levels from ZIKV-infected vs. ZIKV-treated mice. *p < 0.05. In C, differences in weight are displayed as the mean ± SEM, and two-way ANOVA for each day was used to assess the significance. In D, survival was statistically assessed by the log-rank (Mantel‒Cox) test. Independent experiments were performed with ten mice/group (n = 30). *P < 0.05. In E, the inflammatory mediators are presented as the mean ± SEM of 14 mock mice, nine mock-treated, 13 ZIKV-infected mice and 27 ZIKV-treated mice. *P < 0.05 mock- versus ZIKV-infected mice. #P < 0.05 ZIKV-infected mice versus A922500 treatment

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