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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Human umbilical cord-derived mesenchymal stem cell transplantation supplemented with curcumin improves the outcomes of ischemic stroke via AKT/GSK-3β/β-TrCP/Nrf2 axis

Fig. 5

AKT/GSK-3β/β-TrCP/Nrf2 pathway was involved in the combined treatment for the MCAO in vivo. AD Representative western blots (A) and quantitative data for the expression of Nrf2 in the nucleus (B) and the downstream effectors of Nrf2 pathway including HO-1 (C) and NQO1 (D). The hUC-MSCs transplantation and curcumin treatment significantly increased the expression of nuclear Nrf2, HO-1 and NQO1. The expression of Nrf2, HO-1 and NQO1 in MCAO + hUC-MSCs + curcumin group was significantly higher than that in the other groups. EG qRT-PCR analysis for Nrf2 (E), HO-1 (F) and NQO1 (G) with different treatments. There were no differences among the MCAO + hUC-MSCs, MCAO + curcumin and the MCAO + hUC-MSCs + curcumin groups in Nrf2 mRNA expression, while combined treatment upregulated the HO-1 and NQO1 expression. HK Representative western blots (H) and quantitative data for the AKT (Ser473) (I), GSK-3β (Ser9) (J), and β-TrCP in the nucleus (K) for the proteins upstream of the Nrf2 pathway. Combined treatment increased the expression of p-AKT (Ser473), p-GSK-3β (Ser9) and suppressed the nuclear β-TrCP expression. Cur curcumin, N = 5; N.S. no significant difference, *P < 0.05, **P < 0.01, ***P < 0.001

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