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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Human umbilical cord-derived mesenchymal stem cell transplantation supplemented with curcumin improves the outcomes of ischemic stroke via AKT/GSK-3β/β-TrCP/Nrf2 axis

Fig. 8

Combined curcumin and hUC-MSCs treatment promoted anti-inflammatory phenotype microglia polarization through AKT/GSK-3β/β-TrCP/Nrf2 pathway in vitro. A Representative images of immunofluorescent staining of classically activated pro-inflammatory microglia (upper) and alternatively activated anti-inflammatory phenotype (lower) of the microglia. The microglial cell-specific marker Iba1 (green), pro-inflammatory microglia marker iNOS (upper) or anti-inflammatory microglia marker Arg1 (lower) (red), and nuclei (blue). B, C Quantitative immunofluorescence of pro-inflammatory with CD86 (B) and anti-inflammatory with CD206 (C) microglia. D–G qRT-PCR analysis of iNOS (D) and CD86 (E) expression for pro-inflammatory phenotype and Arg1 (F) and CD206 (G) expression for anti-inflammatory phenotype. Cur curcumin, scale bars = 20 μm; N = 5; N.S. no significant difference, **P < 0.01, ***P < 0.001

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Journal of Neuroinflammation

ISSN: 1742-2094