Skip to main content
Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Microglia specific deletion of miR-155 in Alzheimer’s disease mouse models reduces amyloid-β pathology but causes hyperexcitability and seizures

Fig. 1

Expression of miR-155 is not detected at 12 months of age in ex vivo microglia from the APP/PS1 mouse model of AD after Cre-recombinase induction. A Experimental and control genotypes and groups. APPswe/PS1dE9 (APP/PS1) mice were crossed with miR-155flx/flx; CX3CR1CreER/+ mice to generate APP/PS1;miR-155flx/flx; CX3CR1CreER/+ (with tamoxifen: APP/PS1 MG miR-155 CKO mice; with corn oil: APP/PS1 MG miR-155 WT) or non-APP littermate controls that allow for conditional miR-155 deletion (Microglia miR-155 CKO). B) Experimental timeline of study. C Gating strategy for microglia isolation (CX3CR1-YFP+/ CD45low cells) using ex vivo-FACS from the adult mouse CNS. miR-155 copy number in microglia from APP/PS1 MG miR-155 CKO mice and APP/PS1 MG miR-155 WT was quantified by qPCR at D) 6 months of age E) and 12 months of age (Stats: One-Way ANOVA with Tukey’s post hoc correction for multiple comparisons (*** = p < 0.0005)

Back to article page

Journal of Neuroinflammation

ISSN: 1742-2094