Fig. 5

Microglia-specific inducible knock-out of miR-155 leads to increased seizure burden in the APP/PS1 mouse model of AD. A Experimental design summary. Mice were implanted with ECoG and EMG electrodes at 7 weeks of age and, after a baseline recording, miR-155 knock-out was induced at 8 weeks. Continuous ECoG recordings were done for 2–5 weeks or until spontaneous death. Representative trace of B baseline and C seizure that resulted in a spontaneous death event. D Spontaneous seizures were identified and manually quantified starting 1-, 2- and 3-week post-miR-155 deletion in microglia. (Stats: Mixed-effects ANOVA with Tukey’s post hoc correction: ****p < 0.0001, ***p < 0.001, *p < 0.01). E Seizure frequency was increased post miR-155 deletion in microglia in the APP/PS1 background (Stats: Wilcoxon rank sum test with continuity correction, ***p < 0.00001). F % epochs (hours) containing inter-ictal spikes, high-amplitude, synchronous spiking observable via cortical ECoG were increased after miR-155 deletion in microglia (Stats: Mixed Linear Model, p < 0.0001, R² = 0.7311)