Fig. 1

DSP-4 treatment increases dopaminergic neuron degeneration in the 6OHDA model of PD. A Representative images of the SN showing dopaminergic neurons degeneration in the injected SN (TH, white) and B quantification of TH+ neurons in coronal sections of the SN 4 weeks after 6OHDA injection of WT mice treated with Saline, DSP-4 or Desipramine + DSP-4. C NE and serotonin tissue levels measured by HPLC-ED in the cortex and SN of WT mice 4 weeks after DSP-4 or saline treatment. D Representative images of the LC showing noradrenergic neurons (TH, cyan) and quantification of TH+ noradrenergic neurons in coronal sections of the LC 4 weeks after DSP-4 or saline injection in WT mice. Data are shown as mean values ± SEM, each dot represents one mouse, N.S: not significant, *p < 0.05; **p < 0.01; ***p < 0.001. One-way ANOVA followed by Tukey post hoc test; two-tailed t test. Scale bar = 500 µm