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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Astrocytes display ultrastructural alterations and heterogeneity in the hippocampus of aged APP-PS1 mice and human post-mortem brain samples

Fig. 5

Immunostaining for GFAP in typical and dark astrocytes of the stratum lacunosum-moleculare. Representative 5 nm per pixel and 1 nm per pixel of resolution scanning electron microscopy images showing a typical (A) and dark astrocyte (B, C) immunostained with glial fibrillary acidic protein (GFAP) in the ventral hippocampus CA1 stratum lacunosum-moleculare of 20-month-old APP-PS1 male mice. In A a typical astrocyte, denoted by its electron-lucent cyto- and nucleoplasm, is immunopositive for GFAP. In B an electron-dense dark astrocyte with hyper-ramifications and several tertiary lysosomes is immunostained for GFAP. In C a close-up of the dark astrocyte where the GFAP staining is indicated with an orange arrow. Yellow outline = nuclear membrane, purple outline = dark astrocytic cytoplasm, red outline = typical astrocytic cytoplasm, orange arrow = GFAP immunostaining in dark astrocyte, pink pseudo-coloring = dystrophic neurites, purple pseudo-coloring = amyloid beta plaques, 3rd = tertiary lysosomes

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