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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Astrocytes display ultrastructural alterations and heterogeneity in the hippocampus of aged APP-PS1 mice and human post-mortem brain samples

Fig. 6

Density of dark and typical astrocytes in the stratum lacunosum-moleculare. Representative 25 nm per pixel (A) and 5 nm per pixel (B, C) of resolution scanning electron microscopy images of dark astrocytes associated with blood vessels (B) and with the parenchyma (C) from a 20-month-old APP-PS1 male mouse. Quantitative graphs represent the astrocytic density defined ultrastructurally (e.g., via their intermediate filaments, angular processes) and their electron-dense ultrastructure (dark) or electron-lucent (typical) appearance (D) in 20-month-old C57BL/6J vs APP-PS1 male mice. Typical and dark astrocytes in these mice were further categorized based on their association (E) or lack of association (F) with blood vessels in the plane of view. The ratio of dark astrocytic cells associated with a blood vessel and overall astrocytes (typical and dark) associated with the vasculature is represented (G), while the ratio of all dark astrocytes not associated with blood vessels over all astrocytes not associated with blood vessels (typical and dark) is provided (H). Data are shown as individual dots and are expressed as mean ± S.E.M using a Welsh test. Statistical tests were performed on n = 4 mice/group (2–6 levels per animal). Green pseudo-coloring = dark astrocyte associated with blood vessels, purple pseudo-coloring = dark astrocyte not associated with blood vessels

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