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Table 4 Relative ultrastructural analysis of typical astrocytes near vs far from Aß plaques/dystrophic neurites in aged APP-PS1 mice compared to age-matched C57BL/6 mice in the stratum lacunosum-moleculare of the ventral hippocampus CA1

From: Astrocytes display ultrastructural alterations and heterogeneity in the hippocampus of aged APP-PS1 mice and human post-mortem brain samples

 

C57BL/6J

APP-PS1

Control

Mean ± SEM

Far AD

Mean ± SEM

Near AD

Mean ± SEM

Primary lysosomes (%)

39.47 ± 8.036

33.33 ± 8.333

41.94 ± 9.009

Secondary lysosomes (%)

50.00 ± 8.220

36.36 ± 8.504

45.16 ± 9.086

Tertiary lysosomes (%)

31.58 ± 7.642

30.30 ± 8.124

29.03 ± 8.287

Lipid bodies (%)

55.26 ± 8.174

51.52 ± 8.835

61.29 ± 8.893

Altered mitochondria (%)

55.26 ± 8.174

42.42 ± 8.737

61.29 ± 8.893

Elongated mitochondria (%)

84.21 ± 5.995

81.82 ± 6.818

77.42 ± 7.634

Glycogen granules (%) ****

7.895 ± 4.433

12.12 ± 5.770

64.52 ± 8.736 &&&& !!!!

Dilated ER (%)

71.05 ± 7.456

63.64 ± 8.504

80.65 ± 7.213

Nuclear indentation (%)

13.16 ± 5.557

3.030 ± 3.030

19.35 ± 7.213

  1. % percent, a.u. arbitrary unit, ER endoplasmic reticulum, p-values of statistically significant tests are highlighted with various symbols (!, &) with * indicating p value summary
  2. Data reported are shown as % of cells positive for at least one of the elements analyzed for each category and expressed as means ± SEM
  3. ****p < 0.0001 using a Kruskal–Wallis test with Dunn’s multiple comparisons post hoc test. *p value summary, ! Near vs Far AD, & Far AD vs C57BL/6J. Statistical tests were performed on n = 8–12 astrocytes per animal in N = 3 mice/group, for a total of 102 cell bodies analyzed