Fig. 5

Effects of DMHCA on microglia after RIR and Ninj1 activation. a UMAP clustering of myeloid cells. b Violin plot showing the expression of the marker genes for each cell type. c Bar plots showing cell abundances across myeloid for the sham, RIR, and RIR + DMHCA groups. d Dot plot showing the DEGs between the microglia of the RIR vs. sham and RIR + DMHCA vs. RIR comparison groups. Red, blue, and grey dots indicate the up-regulated, downregulated, and unchanged DEGs, respectively. e, f Representative GO and KEGG pathway analyses of up-regulated DEGs of microglia in the RIR vs. sham comparison group and downregulated DEGs of microglia in the DMHCA vs. RIR comparison group. Red and blue bars indicate up- and downregulated DEGs, respectively. g Heatmap showing the expression of enriched genes matched to the GO term inflammatory response. h Venn diagram showing the intersection of the genes enriched in GO terms related to inflammation from the previous GO and KEGG pathway analyses of up-regulated DEGs of microglia in the RIR vs. sham comparison group. i Violin plot showing the expression of Ninj1 in each group. j Western blot analysis of the indicated proteins, Ninj1; protein levels were normalized to GAPDH levels. k Transcriptional levels of Ninj1 in retinas were detected using qRT-PCR; n = 7