Fig. 7From: N,N-Dimethyl-3β-hydroxycholenamide attenuates neuronal death and retinal inflammation in retinal ischemia/reperfusion injury by inhibiting Ninjurin 1Overexpressed Ninj1 reverted the anti-inflammation effect of DMHCA via regulating NF-κB pathway in vitro. a, b Immunofluorescence of TNFα and relative fluorescence intensity among groups (n = 3). Scale bar: 25 μm. c The mRNA expression levels of TNFα, IL-1β and IL6 in BV2 cells were measured with RT-qPCR (n ≥ 3). d, e, f Representative Western blots of apoptosis markers (Clv-casp8), Ninj1, NF-kB pathway markers expressions in BV2 cells. g Cartoon representation, overlay of the crystal structures of small molecule compound DMHCA and its targets Ninj1 were illustrated. h Molecular docking verification of DMHCA and Ninj1. Three-dimensional structures and hydrogen bond of the binding pockets were showedBack to article page