Fig. 1

Expression of NLRC5 responses to various stimuli in vivo and in vitro. A Transcriptions of Nlrc5 in the striatum and the midbrain at 3 days after NS or MPTP administration. B, C Protein levels of NLRC5 in the striatum and the midbrain detected by Western Blot at 7 days after NS or MPTP administration. D Transcriptions of Nlrc5 in primary astrocytes treated with 1 mM MPP⁺ for 0 h, 12 h or 24 h. E, F Protein levels of NLRC5 in primary astrocytes treated with 1 mM MPP⁺ for 0 h, 12 h or 24 h. G Transcriptions of Nlrc5 in primary microglia treated with 100 ng/mL LPS for 0 h or 6 h. H, I Protein levels of NLRC5 in primary microglia treated with 100 ng/mL LPS for 0 h, 6 h or 12 h. J Transcriptions of Nlrc5 in primary neurons treated with PBS or 20 μM MPP⁺ for 24 h. K Transcriptions of Nlrc5 in primary neurons treated with conditioned medium (CM) or LPS-treated conditioned medium (LCM) from mixed glial cells for 24 h. Statistical analyses were performed with Student’s t test (A, C, G, J, K), or one-way ANOVA followed by Holm–Sidak’s multiple comparisons test (D, F, I). n = 3–9. *p < 0.05, **p < 0.01, and ***p < 0.001