Fig. 2

Nlrc5 deficiency ameliorates MPTP-induced dopaminergic neuronal damage in the nigrostriatal axis 7 days after MPTP administration. A HPLC assays of DA (i), DOPAC (ii), and HVA (iii) presented by relative values, the ratio of DOPAC and HVA to DA were calculated (iv, v). n = 6–11. B, C Immunoblotting analysis of TH proteins in the striatum. n = 4–5. D, E Immunohistochemistry staining and optical density analysis of TH⁺ nerve fibers in the striatum. Scale bar, 200 μm. F, J Immunohistochemistry staining and stereological counting of TH⁺ neurons in the SNpc. Scale bar, 200 μm. n = 4. ^#p < 0.05, ** or ^##p < 0.01, and ***p < 0.001