Fig. 4

Assessments of glial activation in the nigrostriatal pathway of WT and Nlrc5^−/− mice at 7 days after MPTP administration. A, B Immunohistochemical staining and counting of Iba1⁺ cells in the striatum. Scale bar, 50 μm. C, D Soma size of Iba1⁺ cells in the striatum. Scale bar, 20 μm. E, F Immunohistochemical staining and counting of GFAP⁺ cells in the striatum. Scale bar, 50 μm. G, H GFAP protein levels in the striatum detected by Western-Blot. I Immunofluorescence double staining of TH (red) and Iba1 (green) in the substantia nigra. Scale bar, 200 μm. J Immunofluorescence double staining of TH (red) and GFAP (green) in the substantia nigra. Scale bar, 200 μm. K Counting of Iba1⁺ cells in the SNpc. L Counting of GFAP⁺ cells in the SNpc. All data were presented as the means ± SEM. n = 4. ^#p < 0.05, ^##p < 0.01, and *** or ^###p < 0.001