Fig. 6
Inflammatory molecules expression in astrocytes, microglia and mixed glial cells treated with neuroinflammatory stimuli. A Transcriptions of IL-1β, IL-6, COX2, iNOS, IFN-α, MHC I, MHC II, IL-10 and TGF-β in enhanced primary astrocytes culture detected by RT-qPCR 24 h after 1 mM MPP+ stimulation. n = 3–6. B Transcriptions of IL-1β, IL-6, COX2, iNOS, C3, MHC I, MHC II, IL-10 and TGF-β in enhanced primary astrocytes culture detected by RT-qPCR 24 h after LPS-induced BV2 conditioned medium (B-LCM) stimulation. n = 4. C Transcriptions of IL-1β, IL-6, TNF-α, COX2, iNOS, NOX1, NOX2, MHC I, MHC II, CIITA, IL-1α, NLRP3, CD40, IFN-β, IL-4 and IL-10 in enhanced primary microglia culture detected by RT-qPCR 6 h after 100 ng/mL LPS stimulation. n = 3–7. D–F Protein levels of COX2 and IL-1β detected by immunoblotting. G Concentration of IL-1β proteins in the supernatants of mixed glial cells measured by ELISA. H Concentration of NO2− in the supernatants of mixed glial cells. I Cell viability of SH-SY5Y cells treated with the supernatants from PBS- or LPS-stimulated WT or Nlrc5−/− mixed glial cells. All data were presented as the means ± SEM. n = 3–6. #p < 0.05, ** or ##p < 0.01, *** or ###p < 0.001