Fig. 8

MPP⁺ and LCM-induced cell death and neuronal survival-related molecule expression in primary neurons. A Representative PI staining and imaging of primary neurons treated with 20 μM MPP⁺ for 24 h. Scale bar, 50 μm. B Counting and statistical analyses of PI⁺ neurons. C Representative PI staining and imaging of primary neurons treated with CM or LCM for 24 h. Scale bar, 50 μm. D Counting and statistical analyses of PI⁺ neurons. E Transcriptions of apoptosis-related molecules Bcl-2 (i), BAX (ii) detected by RT-qPCR and ratio of Bcl2/BAX (iii) in primary neurons treated with 20 μM MPP⁺ for 24 h. F Transcriptions of oxidative stress-related molecules COX2. G–I Protein levels of p-P65 and p-AKT in primary neurons treated with 20 μM MPP⁺ for 24 h detected by immunoblotting. J Transcriptions of apoptosis-related molecules Bcl-2 (i), BAX (ii) detected by RT-qPCR and ratio of Bcl2/BAX (iii) in primary neurons treated with CM or LCM for 24 h. L–N Protein levels of p-P65 and p-AKT in primary neurons treated with CM or LCM for 24 h detected by immunoblotting. All data were presented as the means ± SEM. n = 3–6. * or ^#p < 0.05, ** or ^##p < 0.01, and *** or ^###p < 0.001