Fig. 4

Visualization and analysis of T cell immunoreactivity hαSYN and empty vector (EV) mice. a Representative RGB color images of immunohistochemical staining against CD8⁺ T cells. Scale bars: 250 µm. b Bar plot of counting analysis of CD4⁺ T cells in the substantia nigra (SN). Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: F(3,36) = 6.381, P = 0.0014, n = 10 mice/group. c Bar plot of counting analysis of CD8⁺ T cells in the SN. Statistical analysis by one-way ANOVA followed by Tukey’s multiple comparisons test: F(3,36) = 6.487, P = 0.0013, n = 10 mice/group. d Representative example 8-bit images of immunofluorescence (IF) co-staining against CD8 and NLRP3; Scale bars = 150 µm. e, f Representative example 8-bit images of IF co-staining against CD4 and NLRP3. Overview (left): Scale bars = 150 µm; detail in f: Scale bars: 50 µm. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are shown as mean ± standard error of the mean