Fig. 2

Inhibition of AhR restrains remyelination in cuprizone model. A Time course of CH223191 administration in cuprizone model. B Body weight of control mice, cuprizone-treated mice and mice with CH223191 administration (n = 8 mice per group). Data are shown as mean ± SEM and analyzed by two-way ANOVA with Tukey’s multiple comparisons test. C Beam walking test and rotarod test evaluating motor coordinative function were performed in 3 experimental groups (n = 8 mice per group). Data are shown as mean ± SEM and analyzed by two-way ANOVA with Tukey’s multiple comparisons test. D Western blot analysis of MBP protein level in the corpus callosum of control mice, mice in cuprizone model and mice with CH223191 administration. 25 μg protein was loaded per well. E The quantification of MBP protein expression in D (n = 3 mice per group). Data are shown as mean ± SEM and analyzed by one-way ANOVA with Tukey’s multiple comparisons test. F Representative confocal immunofluorescent images displaying MBP expression in the corpus callosum of 3 experimental groups. Scale bar: 40 μm. G Representative confocal immunofluorescent images of CC1 staining in the corpus callosum of 3 experimental groups. Scale bar: 40 μm. H Representative LFB stained images in the corpus callosum of control mice, mice in cuprizone model and mice with CH223191 administration. Scale bar: 40 μm. *p < 0.05, **p < 0.01, ***p < 0.001. The results of this figure are acquired at the end of week 6 of the cuprizone model