Fig. 4
AhR deficiency in microglia inhibits remyelination. A Schematic of tamoxifen treatment and experimental timeline. B Body weight of AhRfl/fl mice and CX3CR1-AhR−/− mice during demyelination (n = 8 mice per group). Data are shown as mean ± SEM and analyzed by two-way ANOVA with Tukey’s multiple comparisons test. C Beam walking test and rotarod test evaluating motor coordinative function were performed in AhRfl/fl mice and CX3CR1-AhR−/− mice (n = 8 mice per group). Data are shown as mean ± SEM and analyzed by two-way ANOVA with Tukey’s multiple comparisons test. D Representative western blot analysis of MBP protein level in the corpus callosum of AhRfl/fl mice and CX3CR1-AhR−/− mice. 25 μg protein was loaded per well. E The quantification of MBP protein expression in D (n = 4 mice per group). Data are shown as mean ± SEM and analyzed by two-way ANOVA with Tukey’s multiple comparisons test. F Representative LFB stained images in the corpus callosum of AhRfl/fl mice and CX3CR1-AhR−/− mice at the end of week 6 of the cuprizone model. Scale bar: 40 μm. G Representative EM images of the ultrastructure in the corpus callosum of AhRfl/fl mice and CX3CR1-AhR−/− mice at the end of week 6 of the cuprizone model. Scale bar: 5 μm. H The quantification for proportion of myelinated axons in G (n = 3 mice per group). Data are shown as mean ± SEM and analyzed by two-way ANOVA with Tukey’s multiple comparisons test. I Representative confocal immunofluorescent images displaying MBP expression in the corpus callosum of AhRfl/fl mice and CX3CR1-AhR−/− mice with or without I3S treatment. Scale bar: 40 μm. J Representative confocal immunofluorescent images of CC1 staining in the corpus callosum of AhRfl/fl mice and CX3CR1-AhR−/− mice with or without I3S administration. Scale bar: 40 μm. *p < 0.05, **p < 0.01, ***p < 0.001. The results of this figure are acquired in the context of the end of week 6 in the cuprizone model