Fig. 5

Microglial AhR deficiency induces a distinct transcriptional profile in demyelinating condition. A Heatmap of differential gene transcripts in WT mice and CX3CR1-AhR^−/− mice after 5 weeks of cuprizone administration (n = 3 mice per group; fold change > 1.5, adjusted p < 0.05). B tSNE projection of 4 experimental groups. C Volcano plot depicting upregulated and downregulated genes and number of genes upregulated and downregulated in WT mice owing to cuprizone treatment (n = 3 mice per group; fold change > 1.5, adjusted p < 0.05). D Volcano plot depicting upregulated and downregulated genes and number of genes upregulated and downregulated in CX3CR1-AhR^−/− mice owing to cuprizone treatment (n = 3 mice per group; fold change > 1.5, adjusted p < 0.05). E Venn Diagram of differential genes unique to WT mice and CX3CR1-AhR^−/− mice (n = 3 mice per group; fold change > 1.5, adjusted p < 0.05). F KEGG pathway analysis of 473 upregulated genes unique to WT mice after demyelination. G GO term enrichment analysis of 473 upregulated genes unique to WT mice after demyelination. H–J Heatmap of differential transcripts associated with lysosome, phagosome and phagocytosis (n = 3 mice per group; fold change > 1.5, adjusted p < 0.05)