Fig. 12

Pretreatment with repetitive SGB prevents the development of mechanical allodynia and anxiodepressive-like behaviors in ITC rats. A The experimental timeline of surgical procedure and behavior tests. B Temporal changes of PWMT in contralateral hindpaw after thalamic hemorrhagic stroke and the area under curve of the contralateral hindpaw PWMT (n = 8, PWMT: group, F_3,28 = 9.268, day, F_4,112 = 10.48, group × day, F_12,112 = 6.246, *p < 0.05, **p < 0.01 vs ITC + Veh, ^###p < 0.001 vs ITS + Veh; AUC: F_3,28 = 18.74, ^#p < 0.05, ^##p < 0.01). C Temporal changes of PWMT in ipsilateral hindpaw after thalamic hemorrhagic stroke and the area under curve of the ipsilateral hindpaw PWMT (n = 8, PWMT: group, F_3,28 = 10.48, day, F_4,122 = 17.65, group × day, F_12,112 = 7.005, *p < 0.05, **p < 0.01 vs ITC + Veh, ^###p < 0.001 vs ITS + Veh; AUC: F_2,21 = 19.21, ^##p < 0.01). D Representative track plot in the EPMT. E Pretreatment with repetitive SGB increased the time spent and traveled distance in the open arm in ITC group, but had no effect on ITS group (n = 8, time in open arm: F_3,28 = 35.13, ^###p < 0.001; distance in open arm: F_3,28 = 51.39, ^###p < 0.001; total distance: ns, no significance). F Representative track plot in the OFT. G Pretreatment with repetitive SGB increased the time spent and traveled distance in central area in ITC group, but had no effect on ITS group (n = 8, time in central area: F_3,28 = 11.38, ^##p < 0.01, ^###p < 0.001; distance in central area: F_3,28 = 14.80, ^###p < 0.001; total distance: ns, no significance). H Pretreatment with repetitive SGB decreased the latency to feed in the NSFT in ITC group, but had no effect on ITS group (n = 8, W_3.0,15.05 = 23.77, ^#p < 0.05, ^###p < 0.001). I Pretreatment with repetitive SGB decreased the immobility time in the FST in ITC group, but had no effect on ITS group (n = 8, F_2,21 = 8.253, ^##p < 0.01). Data are expressed as mean ± SEM, one-way or two-way ANOVA followed by Tukey test, Welch ANOVA followed by Tamhane’s T2 test