Fig. 4

Intracranial xenograft formation assay to clarify the tumor-promoting function of MS4A7-s in vivo. A The flow diagram of intracranial tumorigenesis experiment. HMC3 cells of EV-control, MS4A7-s OE and MS4A7-l OE group were co-injected with U87MG cells with a ratio of 3:4 into the brains of BALB/c immunodeficient (SCID) mice (n = 7 in each group). MRI was applied to assess the volume of tumors 2 weeks after injection and euthanasia was deployed when neurological signs occurred. B Representative MRI images show the tumors of EV-control, MS4A7-s OE and MS4A7-l OE group, respectively. C Quantitative violin plot of xenograft formation assay (n = 7 in each group). D H&E and Ki-67 IHC staining of the slides of tumor-bearing mice in EV-control, MS4A7-s OE and MS4A7-l OE group. E Survival curves of intracranial tumor-bearing mice co-injected with U87MG and EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells. Technical replicates were performed for each group as indicated. Statistical significances were analyzed by one-way ANOVA (C). ns not significant; **P < 0.01