Fig. 5

Transcriptome profile and signaling pathway activity of EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells. A Quantitative histograms of M1 and M2 markers detected by qRT-PCR in EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells. B PCA plot of RNA-seq data of EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells. C KEGG pathway enrichment analysis of up-regulated genes in MS4A7-s OE HMC3 cells compared to EV-control group. D Heatmap of MS4A7-s OE group up-regulated genes that are enriched in PI3K-Akt signaling pathway and TGF-beta signaling pathway. E Western blotting analysis of p-AKT, AKT, p-GSK-3β and GSK-3β expression levels in EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells. GAPDH was used for normalization. F Volcano plots of DEGs between MS4A7-s OE and EV-control group and DEGs between MS4A7-l OE and EV-control group. G Venn diagrams depict overlaps of differential expressing genes in MS4A7-s OE HMC3 cells. H Heatmap depicts overlaps of differential expressing genes in EV-control, MS4A7-s OE and MS4A7-l OE HMC3 cells. (I) Quantitative histograms of DMKN and MYD88 mRNA expression level with qRT-PCR. GAPDH was used for normalization. Data are presented as mean ± SD (n = 3/group). Statistical significances were analyzed by one-way ANOVA (A, I). ns not significant, *P < 0.05; **P < 0.01; ***P < 0.001