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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Microglia sustain anterior cingulate cortex neuronal hyperactivity in nicotine-induced pain

Fig. 5

Inhibition of ACC pyramidal neurons reduces nicotine-induced allodynia and microglia activation. A Schematic timeline of experiment. B Representative imaging of hM4Di viral expression within glutamatergic neurons in the ACC 3 weeks after viral injection. Scale bars, 200 µm (left) and 20 µm (right). C Changes of PWT and PWL in nicotine-treated mice after inhibition of glutamatergic neurons. D Representative imaging and 3D remodeling of Iba-1-labeled microglia in the ACC in response to mCherry or hM4Di in nicotine-treated mice. E Quantification of Iba-1+ number, intensity, soma size and Imaris-based semi-automatic quantification of Iba-1+ microglia morphometry in the ACC. Scale bars, 40 μm (overview) and 10 μm (Zoom and Rendering). Data were presented as mean ± SEM or Median (IQR). *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant. Details of the statistical analyses are presented in Additional file 1: Table S1

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