Fig. 6

Fractalkine signalling mediates ACC neuron-microglia interactions in nicotine-induced allodynia. A qPCR analysis of CX3CL1 mRNA in vehicle- or nicotine-treated mice after 28 days. B Representative imaging of Iba-1-labeled microglia after intra-ACC ACSF or JMS-17-2 in nicotine-treated mice. Scale bars, 40 μm (overview) and 10 μm (Zoom and Rendering). C, D Quantification of Iba-1⁺ number, intensity, soma size and Imaris-based semi-automatic quantification of Iba-1⁺ microglia morphometry in the ACC. E Changes of PWT and PWL in nicotine-treated mice with intra-ACC ACSF or JMS-17-2. F Sample traces (left) and data of action potentials (middle) and summarize of rheobase (right) of intra-ACC ACSF or JMS-17-2. Data were presented as mean ± SEM or Median (IQR). *p < 0.05; **p < 0.01; ***p < 0.001; ns not significant. Details of the statistical analyses are presented in Additional file 1: Table S1