Fig. 1

[3H]PBR28 binding in ME7-prion mice and pharmacological attenuation by JNJ527. A Representative autoradiographs of [3H]PBR28 in NBH (normal brain homogenate), ME7 and ME7 + JNJ527 mice brains (upper panels represent total signal and lower panels represent non-specific signal). B Mean signal intensity binding of [3H]PBR28 for cortex, hippocampus and thalamus from the three treatment groups. Each dot represents an individual mouse’s data from an average of two consecutive sections from each region of interest. Bars represent mean ± standard error of the mean. For ROI placement examples see Additional file 1: Fig. S2A. Statistical differences: significant effect of treatment group (F (2, 20) = 40.14, p < 0.0001) and ROI (F (1.998, 39.96) = 11.90, p < 0.0001) with no significant group × ROI interaction (F (4, 40) = 2.227, p = 0.0833). Data were analysed with a mixed-effects model ANOVA with ROI as within-subject factor and treatment group as between-subject factor followed by post hoc Sidak tests (n = 7–8/group)