Fig. 2

TSPO regional brain expression in ME7-prion mice and pharmacological attenuation by JNJ527. A Representative TSPO immunohistochemistry images of NBH, ME7 and ME7 + JNJ527 mouse brains. B Quantification of percentage area occupied by TSPO staining in brain regions from the three treatment groups. Each single dot represents an individual mouse’s data from the average of 3–4 sections from each region of interest per animal. Bars show mean ± standard error of the mean. For ROI placement examples see Additional file 1: Fig. S2B. Statistical differences: significant effect of treatment group in the cortical (F (2, 20) = 8.110, p = 0.0026), thalamic (F (2,19) = 15.72, p < 0.0001), and hippocampal regions [CA1 (F (2, 20) = 14.63, p = 0.0001), CA3 (F (2, 20) = 8.509, p = 0.0021) and the dentate gyrus (F (2, 20) = 16.30, p < 0.0001)] (Table 1). Data were analysed with a one-way ANOVA followed by post hoc Tukey tests (n = 7–8/group)