Fig. 3

Microglia/macrophages, astrocytes, neurons and TSPO in the dentate gyrus of the hippocampus in NBH, ME7, ME7 + JNJ527 mice. A Confocal photomicrographs from dentate gyrus-immunostained sections. Microglia/macrophages, Iba1 (purple), astrocytes, GFAP (green), neurones, NeuN (cyan) and TSPO (red). B Cell counts expressed as Iba1+ cells, C GFAP+ cells, D NeuN+ cells, E TSPO+ cells from the three treatment groups (NBH, ME7 prion infected mice, and ME7+ treatment with JNJ527). Scale bar = 20 μm. Each single dot represents an individual mouse’s data from one section from each region of interest from an ROI in the dentate gyrus placed as in Additional file 1: Fig. S2B. Error bars represent mean ± standard error of the mean. Statistical differences: significant effect of treatment group on the number of Iba1+ cells (F (2, 18) = 2.606, p = 0.0003), GFAP+ cells (F (2,20) = 1.096, p = 0.0017) and NeuN+ cells (F (2, 16) = 8.395, p < 0.0001). Data were analysed with a one-way ANOVA followed by post hoc Tukey tests (n = 7–8/group)