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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: The cervical lymph node contributes to peripheral inflammation related to Parkinson’s disease

Fig. 1

Tissue structural and fluid dynamics alteration in dCLNs of A53T mice. A Histology of representative dCLNs of WT and A53T mice. Scale bar, 200 μm. B Quantitative analysis of the cross-sectional area of dCLNs of WT and A53T mice. N = 5 independent animals in each group. C Representative fluorescence images of dCLNs of WT and A53T mice immunolabeled with LYVE-1 (red) and CD169 (green). Scale bar, 200 μm. D Quantitative analysis of the LYVE-1 positive area in dCLNs of WT and A53T mice. N = 4 independent animals in WT and N = 6 independent animals in A53T. E Representative fluorescence images of dCLNs of WT and A53T mice immunolabeled with PDPN. Yellow dotted box indicates reticular meshwork, and purple dotted box indicates medullary cord. Scale bar, 200 μm. F Quantitative analysis of the total surface area of PDPN positive reticular meshwork in dCLNs of WT and A53T mice. N = 5 independent animals in WT and N = 4 independent animals in A53T. G Quantitative analysis of the area of medullary cord in dCLNs of WT and A53T mice. N = 5 independent animals in WT and N = 4 independent animals in A53T. H Schematic of the experimental setup for the monitoring of lymph flow in dCLNs. I Representative images of dCLNs in WT and A53T mice from pre-infusion to 10 min, 20 min, 60 min post-infusion. White dotted circle indicates the dCLN. J Quantitative analysis of Qdot605 signal intensity over 80 min in dCLNs (relative to 8 min post-infusion). t Test was performed at signals between WT and A53T mice at 80 min. N = 4 independent animals. Values are means ± S.E.M, t test (D, F, G, and J), Mann–Whitney U test (B). *, P < 0.05; **, P < 0.01; ****, P < 0.0001

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