Fig. 2

Peripheral inflammation in A53T mice. A Quantitative analysis of mRNA levels of Il1b, Il6 and Tnf in dCLNs of WT and A53T mice using qPCR. N = 3 independent animals in each group. B Western blot to assess the level of IL-1β, IL-6 and TNF-α in dCLNs of WT and A53T mice. C Quantitative analysis of the protein level of IL-1β, IL6 and TNF-α in dCLNs of WT and A53T mice, N = 3 independent in each group (3 samples were pooled from 6 animals). D Representative fluorescence images of dCLNs from WT and A53T mice immunolabeled by CD11b (red or green) with IL-1β (green), IL-6 (red), or TNF-α (red). Scale bar, 100 μm. E Quantitative analysis of IL-1β, IL6 and TNF-α positive area in dCLNs of WT and A53T mice. N = 5 independent animals in WT and A53T. F Quantitative analysis of the levels of IL-1β, IL6 and TNF-α using ELISA, in plasma of WT and A53T mice. N = 4 independent animals in WT group and N = 5 independent animals in A53T group. Values are means ± S.E.M, t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001