Fig. 4

Endoplasmic reticulum stress was increased in the dCLN of A53T mice. A Functional enrichment of differentially expressed genes in dCLNs of WT and A53T mice. Red represented the number of increased genes and blue represented the number of decreased genes. B Heat map partly showing the relative expression levels of genes involved in response to endoplasmic reticulum stress signaling pathway. Red represents gene expression levels above the mean; blue represents gene expression levels below the mean. N = 3 independent in each group (3 WT samples were pooled from 6 animals; 3 A53T samples were pooled from 4 animals). C Western blot to assess the level of p-IRE1α, IRE1α, GRP78, ATF6, ATF6-p, ATF4, sXBP-1, p-eIF2α, eIF2α and α-synuclein in dCLNs of WT and A53T mice. D–K Quantitative analysis of the level of IRE1α, p-IRE1α, sXBP-1, ATF6, ATF6-p, p-eIF2α, eIF2α and ATF4 in dCLNs of WT and A53T mice, N = 4 independent in WT group (4 WT samples were pooled from 8 animals) and N = 4 independent animals in A53T group. L Quantitative analysis of Ddit3 mRNA levels using qPCR, in dCLNs of WT and A53T mice. N = 3 independent animals in each group. M Quantitative analysis of the level of GRP78 in dCLNs of WT and A53T mice. Values are means ± S.E.M, t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001