Fig. 7

ER stress was involved in the peripheral inflammation in PD mice. A Quantitative analysis mRNA levels of Il1b, Il6 and Tnf in BMDMs treated with 100 nM α-syn monomer or oligomer and vehicle control or 100 μM TUDCA using qPCR. N = 3 independent experiments in each group. B Quantitative analysis of the levels of IL-1β, IL6 and TNF-α released by BMDMs treated with 100 nM α-syn monomer or oligomer and vehicle control or 100 μM TUDCA using ELISA. N = 3 independent in α-syn oligomer group, N = 6 independent in α-syn oligomer and TUDCA group. C Western blot to assess the level of IL-1β, IL6 and TNF-α in dCLNs of WT or A53T mice treated with vehicle control and TUDCA (100 mg/kg). D Quantitative analysis of the levels of IL-1β, IL6 and TNF-α in dCLNs of WT or A53T mice treated with vehicle control and TUDCA. N = 3 independent in each group (3 WT + Vehicle samples were pooled from 7 animals; 3 WT + TUDCA samples were pooled from 7 animals; 3 A53T + Vehicle samples were pooled from 5 animals; 3 A53T + TUDCA samples were pooled from 6 animals). E Representative fluorescence images of IL-1β, IL6 and TNF-α in dCLNs of A53T mice treated with or without TUDCA. Scale bar, 100 μm. F Quantitative analysis of IL-1β, IL6 and TNF-α positive area in dCLNs of A53T mice treated with or without TUDCA. N = 5 independent animals in each group. Values are means ± S.E.M, one-way ANOVA test (A, B, D), t test (F). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001